Since the early 1990s, FTMS has transformed biomolecular mass spectrometry, but its role in proteomics has been dominated by bottom-up (peptide-based) strategies. Top-down proteomics instead analyzes intact proteins, directly revealing proteoforms (the molecular variants arising from genetic variation, splicing, and post-translational modifications). By capturing the complete biochemical state...
Photoionization schemes have been extensively explored in both vacuum and ambient ionization mass spectrometry. In ambient ionization, atmospheric pressure photoionization (APPI) development has largely stalled, and atmospheric pressure laser ionization (APLI) remains niche. However, photoionization provides unique control over ionization characteristics and structural selectivity based on...
Since the invention of FT-ICRMS 1973 by Comisarow and Marshall, many innovations have been implemented in home built and commercial FT-ICR instrumentation and made available to researchers. With the change of user profile, moving from fundamental expert research to routine applications, more focus is on ease of use, often enabled by technology advances like Maxwell magnet technology and XR and...
High-resolution mass spectrometry is the method of choice for analyzing complex mixtures, as this is the only method that allows gaining detailed analytical information with high accuracy. The only problem is that mass spectrometry inherently can not provide structural information about unknown compounds per se. Liquid fuel systems are some of the most complex natural mixtures available and...
Despite its crucial role in global trade, shipping significantly contributes to environmental pollution by emitting greenhouse gases and pollutants like nitrogen oxides, sulfur oxides, and particulate matter. To mitigate the harmful effects of ship emissions, the International Maritime Organization (IMO) has implemented a global limit on the sulfur content in marine fuels and introduced sulfur...
High-resolution Fourier transform mass spectrometry (FTMS) combined with native electrospray ionization provides direct access to the proteoform and glycoform heterogeneity of intact glycoproteins. As a defined model system, the therapeutic antibodies adalimumab and trastuzumab were analyzed to test the ability of native MS to resolve Fc glycosylation variants with high mass accuracy. In...
